Resolution of the hemes of hydroxylamine oxidoreductase by redox potentiometry and optical spectroscopy

FEBS Letters

Hooper, Alan B.²  Prince, Roger C.³  Larroque, Christian¹ 

[1] INSERM U128, Montpellier 34033, France;Department of Genetics and Cell Biology, University of Minnesota, St. Paul, MN 55108, USA;Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA

关键词: Hydroxylamine oxidoreductase;    Redox potentiometry;    Nitrosomonas;    Cytochrome;   

DOI  :  10.1016/0014-5793(83)81154-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Optical spectroscopy combined with redox potentiometry has resolved the hemes of hydroxylamine oxidoreductase into 6 thermodynamically distinct classes. There are apparently 4 classes of heme c ₅₅₃, with E _m7-values of 295 mV, 10 mV, −190 mV and −390 mV, present in a stoichiometry of 1:1:2:1; two equivalents of heme c ₅₅₉, E _m7 O mV, and one of heme P-460, an unusual chromophore, with E _m7 −260 mV.

【 授权许可】

Unknown