Oxidation of glutathione by free radical intermediates formed during peroxidase‐catalysed N‐demethylation reactions

FEBS Letters

Berggren, Margareta¹  Thor, Hjördis¹  Moldéus, Peter¹  Orrenius, Sten¹  O'Brien, Peter J.¹ 

[1] Department of Forensic Medicine, Karolinska Institutet, Box 60400, S-104 01 Stockholm, Sweden

关键词: Glutathione;    Thiol oxidase;    γ-Glutamyl transferase;    Tubular epithelium;    Renal cell;    Plasma membrane;   

DOI  :  10.1016/0014-5793(83)80797-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Horseradish peroxidase-catalyzed N-demethylation of aminopyrine and dimethylaniline results in generation of free radical intermediates which can interact with glutathione (GSH) to form a glutathione radical. This can either dimerize to yield glutathione disulfide or react with O₂ to form oxygenated products of glutathione. Ethylmorphine is not a substrate in the peroxidase-mediated reaction, and free radical intermediates which react with GSH, are not formed from aminopyrine and dimethylaniline when the horseradish peroxidase/H₂O₂ system is replaced by liver microsomes and NADPH. Therefore, it appears unlikely that formation of free radical intermediates can be responsible for the depletion of GSH observed during N-demethylation of several drugs in isolated liver cells.

【 授权许可】

Unknown