Multiple environments of fluorinated anesthetics in intact tissues observed with 19F NMR spectroscopy

FEBS Letters

Li, Yao-En²  Schofield, John C.³  Tyler Burt, C.¹  Wyrwicz, Alice M.² 

[1] Department of Radiology, Massachusetts General Hospital, MA 02114, USA;Department of Chemistry, University of Illinois at Chicago, Chicago, IL 60680, USA;Biological Resources Laboratory, University of Illinois at Chicago, Chicago, IL 60680, USA

关键词: Halothane;    Methoxyflurane;    Nerve;    Muscle;    Erythrocyte;    NMR;   

DOI  :  10.1016/0014-5793(83)80783-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The incorporation of two fluorine-containing general anesthetic agents, halothane and methoxyflurane, into erythrocytes (from three different species), rabbit muscle and rabbit nerve, was followed with ¹⁹F NMR spectroscopy. Two major findings emerged from these studies: (1) multiple environments indicative of domain structure in the membrane can be observed depending on the anesthetic and the tissue type; and (2) the ¹⁹F chemical shifts of a given anesthetic were characteristic for the tissue examined. Halothane showed a single resonance in erythrocytes and multiple resonances in muscle and nerve, while methoxyflurane showed multiple resonances in both muscle and erythrocytes. The range of the ¹⁹F chemical shifts for the multiple peaks was as great as 6 ppm.

【 授权许可】

Unknown