Soluble phorbol ester binding sites and phospholipid‐ and calcium‐dependent protein kinase activity in cytosol of chick oviduct

FEBS Letters

Kittstein, W.¹  Fürstenberger, G.¹  Horn, F.¹  Gschwendt, M.¹  Marks, F.¹ 

[1] Deutsches Krebsforschungszentrum, Institut für Biochemie, Im Neuenheimer Feld 280, D-6900 Heidelberg, FRG

关键词: Chick oviduct Cytosol TPA binding Protein kinase;   

DOI  :  10.1016/0014-5793(83)81067-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A large amount of specific high affinity binding sites for tumor promoting phorbol esters as well as of a Ca²⁺- and phospholipid-dependent protein kinase is present in cytosol of chick oviduct. 12-O-Tetradecanoyl-phorbol-13-acetate (TPA) is able to replace either Ca²⁺ or the phospholipid phosphatidylserine as activators of the kinase to some extent. The maximum activity of the enzyme in the presence of Ca²⁺ and phosphatidylserine, however, cannot be increased further by TPA. Various second stage tumor promoters also exhibit the capacity to stimulate the protein kinase, whereas the non-promoting phorbol ester 4-O-methyl-TPA, as well as the non-promoting, but with respect to other responses TPA-like, calcium ionophore A23187, do not affect the kinase.

【 授权许可】

Unknown