Neurotransmitter‐caused increase in [3H]inositol incorporation into phosphatidylinositol de novo synthesis vs exchange

FEBS Letters

Lupu, Monica¹  Oron, Yoram¹ 

[1] The Department of Physiology and Pharmacology, The Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Israel

关键词: Phosphatidylinositol αAdrenergic Muscarinic Receptors Rat parotid;   

DOI  :  10.1016/0014-5793(83)81064-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

[³H]inositol and ³²P_i were simultaneously incorporated into rat parotid phosphatidylinositol. The ratio of [³H]/³²P_i incorporation dropped dramatically following stimulation with muscarinic or α-adrenergic agonists and returned to control values following the addition of appropriate antagonists. The drop in [³H]/³²P_i ratio can be explained by a rapid increase in de- novo synthesis of phosphatidylinositol following its receptor-mediated breakdown. The change in this ratio also provided evidence for the existence of CDP-DG + inositol ⇄ phosphatidylinositol exchange reaction in the intact tissue.

【 授权许可】

Unknown