FEBS Letters | |
A higher affinity of AMV reverse transcriptase for template‐primers correlates with a lower rate of DNA synthesis | |
Das, M.R.1  Parnaik, Veena K.1  | |
[1] Centre for Cellular and Molecular Biology, Hyderabad 500007, India | |
关键词: Reverse transcriptase; Template binding; DNA synthesis; AMV; avian myeloblasblastosis virus; | |
DOI : 10.1016/0014-5793(83)80748-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The kinetics of copying of poly (A)-oligo (dT) and poly (C)-oligo (dG) by reverse transcriptase from avian myeloblastosis virus have been studied, and binding affinity of enzyme for template-primer and primer alone have been determined separately. Although the maximal rate of DNA synthesis obtained with poly (C)-oligo (dG) is higher than that for poly (A)-oligo (dT), the binding affinity of the enzyme for poly (C)-oligo (dG) or oligo (dG) is considerably lower than that for poly (A)-oligo (dT) or oligo (dT). Hence, for the more efficient template, poly (C)-oligo (dG), both template-primer and primer bind less tightly to the enzyme.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020284663ZK.pdf | 278KB | download |