A higher affinity of AMV reverse transcriptase for template‐primers correlates with a lower rate of DNA synthesis

FEBS Letters

Das, M.R.¹  Parnaik, Veena K.¹ 

[1] Centre for Cellular and Molecular Biology, Hyderabad 500007, India

关键词: Reverse transcriptase;    Template binding;    DNA synthesis;    AMV;    avian myeloblasblastosis virus;   

DOI  :  10.1016/0014-5793(83)80748-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The kinetics of copying of poly (A)-oligo (dT) and poly (C)-oligo (dG) by reverse transcriptase from avian myeloblastosis virus have been studied, and binding affinity of enzyme for template-primer and primer alone have been determined separately. Although the maximal rate of DNA synthesis obtained with poly (C)-oligo (dG) is higher than that for poly (A)-oligo (dT), the binding affinity of the enzyme for poly (C)-oligo (dG) or oligo (dG) is considerably lower than that for poly (A)-oligo (dT) or oligo (dT). Hence, for the more efficient template, poly (C)-oligo (dG), both template-primer and primer bind less tightly to the enzyme.

【 授权许可】

Unknown