Occurrence of a new hematoside in the kidney of guinea pig

FEBS Letters

Hirabayashi, Yoshia²  Li, Yu-Teh²  Li, Su-Chen¹ 

[1] Delta Regional Primate Research Center, Tulane University, Covington, LA 70433, USA;Department of Biochemistry, Tulane University School of Medicine, New Orleans, LA 70112, USA

关键词: Ganglioside;    O-Acetylhematoside;    Sialic acid;    Guinea pig;    Glycosphingolipid;    Hematoside;    C—M—W;    chloroform—methanol—water;    TLC;    thin-layer chromatography;    GLC;    gas-liquid chromatography;   

DOI  :  10.1016/0014-5793(83)80744-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated a new hematoside from guinea pig kidney. Like the usual hematoside (II³NeuAc-LacCer), isolated from human erythrocytes, this new hematoside contained glucose, galactose, and N-acetylneuraminic acid in an equimolar proportion. By thin-layer chromatography (TLC), however, it migrated faster than the usual hematoside. After mild alkaline hydrolysis the TLC mobility of this ganglioside bacame identical to that of the usual hematoside. The sialic acid in this ganglioside was susceptible to Clostridial neuraminidase. Based on TLC mobility and the results of periodate oxidation, the sialic acid of the new hematoside was identified as 9-O-acetyl-N-acetylneuraminic acid. Therefore, the structure of this new hematoside is 9-O-Ac-NeuAcα2→3Galβ1→4GLcβ1→1′Cer.

【 授权许可】

Unknown