Isolation of a photosystem 2 preparation from higher plants with highly enriched oxygen evolution activity

FEBS Letters

Evans, M.C.W.¹  Ford, R.C.¹ 

[1] Department of Botany and Microbiology, University College London, Gower Street, London WC1E 6BT, England

关键词: Photosystem 2;    Oxygen evolution;    P680+;    Cytochrome b-559;    Signal IILT;    Chloroplast;    chl;    chlorophyll;    DCMU;    3-(3';    4'-diachlorophenyll)-1;    1-dimethylurea;    EPR;    electron paramagnetic resonance;    MES;    2-(N-morpholino) ethanesulfonic acid;    PS2;    photosystem 2;    PS1;    photosystem 1;    Tris;    Tris-(hydroxymethyl)aminomethane;    DMBQ;    2;    6-dimethyl-benzoquinone;   

DOI  :  10.1016/0014-5793(83)80957-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Detergent-treatment of higher plant thylakoids with Triton X-100 at pH 6.3 has been used to purify a PS2 fraction with very high rates of oxygen evolution (1000 μmol.mg chl⁻¹.h⁻¹). A photosynthetic unit size of about 300 chlorophyll (chl) molecules has been determined by optical methods, suggesting an average turnover time for PS2 of about 2 ms. The donor system for P680⁺ is particularly well preserved in the preparation, as judged by P680⁺ reduction kinetics, the detection by EPR of Signal II_LT and the presence of the high potential form of cytochrome b-559 (at a ratio of 1:1 with the reaction centre).

【 授权许可】

Unknown