FEBS Letters | |
Isolation of a photosystem 2 preparation from higher plants with highly enriched oxygen evolution activity | |
Evans, M.C.W.1  Ford, R.C.1  | |
[1] Department of Botany and Microbiology, University College London, Gower Street, London WC1E 6BT, England | |
关键词: Photosystem 2; Oxygen evolution; P680+; Cytochrome b-559; Signal IILT; Chloroplast; chl; chlorophyll; DCMU; 3-(3'; 4'-diachlorophenyll)-1; 1-dimethylurea; EPR; electron paramagnetic resonance; MES; 2-(N-morpholino) ethanesulfonic acid; PS2; photosystem 2; PS1; photosystem 1; Tris; Tris-(hydroxymethyl)aminomethane; DMBQ; 2; 6-dimethyl-benzoquinone; | |
DOI : 10.1016/0014-5793(83)80957-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Detergent-treatment of higher plant thylakoids with Triton X-100 at pH 6.3 has been used to purify a PS2 fraction with very high rates of oxygen evolution (1000 μmol.mg chl−1.h−1). A photosynthetic unit size of about 300 chlorophyll (chl) molecules has been determined by optical methods, suggesting an average turnover time for PS2 of about 2 ms. The donor system for P680+ is particularly well preserved in the preparation, as judged by P680+ reduction kinetics, the detection by EPR of Signal IILT and the presence of the high potential form of cytochrome b-559 (at a ratio of 1:1 with the reaction centre).
【 授权许可】
Unknown
【 预 览 】
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