Ecdysteroid and juvenile hormone changes in Bombyx mori eggs, related to the initiation of diapause

FEBS Letters

Gharib, Bouchra¹  Chaix, Jean-Claude²  Connat, Jean-Louis³  de Reggi, Max¹ 

[1] Centre d'Immunologie, Case 906, 13288 Marseille — Cedex 9, France;Laboratoire de Zoologie Marine, Faculté des Sciences, St Jerôme, 13397 Marseille — Cedex 9, France;Laboratoire de Zoologie, Boulevard Gabriel, 21000 Dijon France

关键词: Ecdysteroid;    Juvenile hormone;    (Bombyx mori egg);    Diapause initiation;    Hormone level changes;    Embryogenesis;   

DOI  :  10.1016/0014-5793(83)80949-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We describe a method for the routine determination of changes in juvenile hormone levels in insect eggs. The hormones are first converted into their diol derivatives, then they are purified from other lipids and separated by high-performance liquid chromatography (HPLC). The radioimmunoassay of the fractions was then determined. The method permits the simultaneous assay of ecdysteroids, and it was used for determining the hormonal changes in Bombyx eggs during the pre-diapause development. Our major finding is that the hormonal content of eggs dramatically increased prior to the initiation of diapause. This hormonal rise included ecdysone, 20-OH-ecdysone and 3 juvenile hormones. The HPLC retention time of the latter corresponded to JH₁ JH₂ and JH₃. Subsequently, the embryos entered diapause and the hormonal content of eggs was reduced to traces of ecdysteroids. These dramatic changes in juvenile hormone levels during early embryogenesis raise a number of issues which are developed in the discussion.

【 授权许可】

Unknown