Cellular RNA is not degraded in interferon‐treated HeLa cells after poliovirus infection

FEBS Letters

Muñoz, Alberto¹  Harvey, Robert²  Carrasco, Luis¹ 

[1] Departamento de Microbiología, Centro de Biología Molecular, U.A.M. and C.S.I.C. Canto Blanco, Madrid 34, Spain;Biochemistry Division, National Institute for Medical Research, Mill Hill, London NW7 1AA, England

关键词: Interferon;    Poliovirus;    Protein synthesis;    Shut-off;    2′–5′ A Synthetase;    RNA degradation;   

DOI  :  10.1016/0014-5793(83)80942-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A drastic inhibition of protein synthesis occurs in HeLa cells treated with human lymphoblastoid interferon and infected with poliovirus. At the time when this inhibition has been established no degradation of ³²P-labelled ribosomal RNA can be detected. Isolation of the mRNAs from poliovirus-infected cells plus or minus interferon treatment, followed by translation in a reticulocyte lysate indicates that cellular mRNAs remain active. These results suggest that gross degradation of cellular RNA does not occur in interferon-treated poliovirus-infected HeLa cells and that a non-specific nuclease induced by 2′–5′ A is not responsible for the inhibition of protein synthesis observed.

【 授权许可】

Unknown