The primary structure of initiation factor IF3 from Bacillus stearothermophilus

FEBS Letters

Kimura, Makoto¹  Appelt, Krzysztof¹  Ernst, Helmut¹ 

[1] Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, D-1000 Berlin-Dahlem 33, Germany

关键词: Bacillus stearothermophilus;    Initiation factor 3;    Protein primary structure determination;    Protein biosynthesis;    Sequence comparison;   

DOI  :  10.1016/0014-5793(83)80940-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complete amino acid sequence of the initiation factor IF3 from Bacillus stearothermophilus has been elucidated. This was achieved by splitting the protein with trypsin, Staphylococcus protease or cyanogen bromide. The amino acid sequence was determined by manual Edman degradation, using the DABITC/PITC double-coupling method. The IF3 molecule contains 171 amino acids and has an M _r of 19 677. The sequence was compared to the homologous molecule from Escherichia coli; about 50% of the amino acid residues were found to be identical.

【 授权许可】

Unknown