Enzymatic conversion of apolar compounds in organic media using an NADH‐regenerating system and dihydrogen as reductant

FEBS Letters

Hilhorst, Riet¹  Laane, Colja¹  Veeger, Cees¹ 

[1] Department of Biochemistry, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands

关键词: Enzymatic steroid conversion;    Reversed micelle;    NADH regeneration;   

DOI  :  10.1016/0014-5793(83)80451-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A combined enzyme system, consisting of hydrogenase, lipoamide dehydrogenase and 20β-hydroxysteroid dehydrogenase has been enclosed in reversed micelles. This system catalyzes the stereo- and site-specific enzymatic reduction of apolar, poorly water-soluble ketosteroids to their corresponding 20β-hydroxyform using an in situ NADH-regenerating enzyme system and H₂ as ultimate reductant.

【 授权许可】

Unknown