Difference between tortoise and hen lysozymes in the mode of association with N‐acetyl D‐glucosamine as revealed by proton magnetic resonance spectroscopy

FEBS Letters

Chatterjee, N.¹  Banerjee, Kalyan K.²  Sen, A.² 

[1] Saha Institute of Nuclear Physics, 92, Acharya Prafulla Chandra Road, Calcutta 700 009, India;Department of Chemistry, Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta 700 009, India

关键词: Lysozyme;    association;    N-Acetyl D-glucosamine;    PMR;    Chemical shift;    (tortoise;    hen);   

DOI  :  10.1016/0014-5793(83)80440-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tortoise lysozyme, unlike hen lysozyme, does not distinguish between the α- and β-anomeric forms of N-acetyl D-glucosamine. This indicates that α- and β-anomeric forms of the inhibitor have the same affinities and experience identical magnetic environments in tortoise lysozyme. The dissociation constant of tortoise lysozyme—inhibitor complex was calculated from chemical shift data and found to be 3.5 × 10⁻² M. The enthalpy of dissociation was calculated to be 5.0 kcal/mol.

【 授权许可】

Unknown