Stable isotope containing peptides as probes of ligand—receptor interactions

FEBS Letters

Muthukumaraswamy, Natesa¹  Glasel, Jay A.³  Freer, Richard J.¹  Wu, Arthur¹  Becker, Elmer L.²  Mackin, William M.² 

[1] Department of Pharmacology, Medical College of Virginia, Richmond, VA, 23298, USA;Department of Pathology, University of Connecticut Health Center, Farmington, CT 06032, USA;Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032, USA

关键词: Chemotactic peptide;    Lysosomal enzyme release;    Receptor;    Deuterium;    Hydrogen-bonding;   

DOI  :  10.1016/0014-5793(83)80435-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The synthesis and biological evaluation of the stable isotope containing chemotactic peptide, deuteroformyl-methionyl-leucyl-phenylalanine (C²DO-Met-Leu-Phe-OH) is reported. The results, using lysosomal enzyme release from cytochalasin B-treated rabbit neutrophils for biological evaluation indicates a 3–4 fold enhancement over CHO-Met-Leu-Phe-OH itself. This is consistent with previous speculation that the formyl proton hydrogen bonds with a critical area of the chemotactic peptide receptor of rabbit neutrophils.

【 授权许可】

Unknown