The photoaffinity probe 8‐N3[α‐32P]ATP labels the ATP‐binding sites of rabbit neutrophil and skeletal muscle actin

FEBS Letters

Bormann, Barbara-Jean¹  Hill, John M.¹  Huang, Chi-Kuang¹  Becker, Elmer L.¹  Mackin, William M.¹ 

[1] Department of Pathology, University of Connecticut Health Center, Farmington, CT 06032, USA

关键词: 8-N3-ATP;    Actin;    Photoaffinity labeling;    8-N3-ATP;    8-azido-adenosine-5'-triphosphate;    SDS;    sodium dodecyl sulfate;    EGTA;    ethylene glycol bis (β-aminoethyl ether)-N;    N;    N';    N'-tetracetic acid;   

DOI  :  10.1016/0014-5793(83)80434-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

8-Azido-[α-³²P]ATP (8-N₃-ATP) was used as a photoaffinity label for ATP binding sites in the subcellular fractions of rabbit peritoneal neutrophils. The radioactive 8-N₃-ATP was specifically incorporated into one major protein of 43 kDa. The isoelectric point, molecular mass and subcellular distribution of this labeled protein closely resemble those of the actin. 8-N₃-[α-³²P]ATP was further tested as a photoaffinity label for the ATP binding site in the purified rabbit skeletal muscle G-actin. The radioactive 8-N₃-ATP was specifically incorporated into the actin band in SDS—polyacrylamide gel. The results indicate that 8-N₃-ATP can be used as a photoaffinity label for actin.

【 授权许可】

Unknown