Immunochemical evidence for the presence of 5mC, 6mA and 7mG in human, Drosophila and mealybug DNA

FEBS Letters

Achwal, Chhaya W.¹  Chandra, H.Sharat¹  Iyer, Chitra A.¹ 

[1] Microbiology and Cell Biology Laboratory, ICMR Centre for Genetics and Cell Biology, Indian Institute of Science, Bangalore 560 012, India

关键词: DNA methylation;    Biotin—avidin cross-linking;    DNA—protein interaction;    Gene regulation Z-DNA;    X-chromosome inactivation;   

DOI  :  10.1016/0014-5793(83)80612-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have reported that production and characterization of antibodies highly specific to 5-methyl-cytosine (5mC) and the development of a sensitive immunochemical method for the detection of 5mC in DNA [FEBS Lett. (1982) 150, 469]. Extension of this method to two other modified bases, 6-methyladenine (6mA) and 7-methylguanine (7mG), is reported here. By use of this immunochemical approach, we are able to detect 5mC, 6mA and 7mG in human and Drosophila DNA and confirm their presence in the DNA of two mealybug species.

【 授权许可】

Unknown