Collagen of fibrocartilage: a distinctive molecular phenotype in bovine meniscus

FEBS Letters

Wu, J.J.¹  Eyre, David R.¹ 

[1] Children's Hospital and Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA

关键词: Bovine;    Cartilage;    Collagen;    Fibrocartilage;    Meniscus;    CNBr;    cyanogen bromide;    TFA;    trifluoroacetic acid;    EDTA;    ethylenediamine tetraacetic acid;   

DOI  :  10.1016/0014-5793(83)80592-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fibrocartilage of bovine knee meniscus was analyzed for major and minor collagen constituents. The main fraction (∼98%) of pepsin-solubilized collagen consisted of type I with a small proportion (< 10%) of type III molecules. The minor fraction (1–2%) isolated by salt precipitation could be further resolved into type V collagen that consisted of α1(V) and α2(V) chains and a type II-like molecule with chains that had all the characteristics of the 3α variant of α1(II) found in hyaline cartilage. The articular surface zone of the meniscus appeared 2–3-fold enriched in these minor collagens compared with deeper tissue, though qualitatively the same distinctive collagen phenotype was evident throughout.

【 授权许可】

Unknown