An artificial crystalline DDT‐binding polypeptide

FEBS Letters

Gutte, Bernd¹  Thomas, Richard M.¹  Moser, Rudolf¹ 

[1] Biochemisches Institut der Universität Zürich, Zürichbergstrasse 4, CH-8028 Zürich, Switzerland

关键词: Protein secondary structure prediction;    Model building;    Protein design;    Synthetic DDT-binding polypeptide;    Crystallization;    of artificial polypeptide;    DDT;    2;    2-bis(p-chlorophenyl)-1;    1;    1-trichloroethane;   

DOI  :  10.1016/0014-5793(83)80555-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A hydrophobic 24-residue polypeptide that could potentially form a four-stranded antiparallel β-pleated sheet and bind the insecticide DDT was designed and synthesized. The synthetic peptide aggregated in 1 M acetic acid but was monomeric in aqueous 50% ethanol. In the latter solvent the 24-residue polypeptide and DDT formed a complex with an apparent dissociation constant of ≈2 × 10⁻⁵ M. The DDT binding of an analogue of this peptide possessing the same amino acid residues in a random sequence was more than 2 orders of magnitude and that of bovine serum albumin at least 3 orders of magnitude weaker. The designed polypeptide could be crystallized.

【 授权许可】

Unknown