The effect of re‐oxidation on the reduced hydrogenase of Desulfovibrio vulgaris strain Hildenborough and its oxygen stability

FEBS Letters

Dijk, Cees van¹  Berkel-Arts, Anita van¹  Veeger, Cees¹ 

[1] Department of Biochemistry, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands

关键词: Hydrogenase;    Desulfovibrio vulgaris;    Re-oxidation;    Oxygen stability;   

DOI  :  10.1016/0014-5793(83)80525-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Upon reduction of the oxygen-stable hydrogenase of Desulfovibrio vulgaris strain Hildenborough this enzyme becomes oxygen sensitive. Only anaerobic oxidation of the reduced enzyme with 2,6-dichlorophenol-indophenol, in the presence of iron and EDTA, followed by addition of Tris-EDTA (pH 9.0) converts the enzyme back into its oxygen-stable state, without loss of activity.

【 授权许可】

Unknown