Simultaneous isolation of the yeast cytosol and well‐preserved mitochondria with negligible contamination by vacuolar proteinases

FEBS Letters

Flores, Alberto¹  Schwencke, Jaime¹  Canut, Hervé¹ 

[1] Laboratoire d'Enzymologie du CNRS, 91190 Gif-sur-Yvette, France

关键词: Yeast Proteinase Vacuole Cytoplasm Proteolysis Mitochondria;   

DOI  :  10.1016/0014-5793(83)80512-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Disruption of yeast spheroplasts by DEAE-dextran in isoosmotic conditions allows isolation of relatively undamaged subcellular fractions from yeast. The preservation of mitochondria and vacuoles permits the simultaneous isolation of the cytosol with negligible contamination by vacuolar proteinases and therefore, virtually eliminates proteolytic artefacts.

【 授权许可】

Unknown