Norepinephrine‐induced loss of phosphatidylinositol from isolated rat liver plasma membrane

FEBS Letters

Wallace, Michael A.¹  Claret, Michel¹  Poggioli, Josiane¹  Giraud, Françoise² 

[1] Laboratoire de Physiologie Comparée, LA 89, Bat 443, Université Paris Sud, 91405 Orsay, Cedex, France;Laboratoire de Physiologie de la Nutrition, U 231, ERA 415, Bat 447, Université Paris-Sud, Orsay Cedex, France

关键词: Noradrenaline;    Phosphatidylinositol;    Ca2+;    Mg2+;    Isolated plasma membranes;    PtdIns;    phosphatidylinositol;    NE;    1-norepinephrine;    EGTA;    ethyleneglycol-bis (β-aminoethyl ether-N;    N'-tetraacetic acid;   

DOI  :  10.1016/0014-5793(83)80504-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Norepinephrine at 5 μM induces a rapid (60 s) and specific loss of phosphatidylinositol (PtdIns) when added to isolated rat liver plasma membranes. The hormone action is inhibited by the α-adrenergic antagonist phentolamine (20 μM). Depletion of Mg²⁺ and Ca²⁺ singly or in combination from the incubation buffer mimicks the hormone effect on PtdIns breakdown. No further effect on PtdIns degradation could be measured when norepinephrine was added to the cation-depleted buffers. Addition of the Ca²⁺ ionophore A23187 to the isolated membranes has no effect. It is concluded that PtdIns degradation can be provoked in isolated rat liver plasma membrane through α-adrenergic receptor activation and that this effect is dependent on divalent cations in the sense that loss of cations from the membrane allows degradation to commence.

【 授权许可】

Unknown