Isolation of oxygen‐evolving phycobilisome—photosystem II particles from Porphyridium cruentum

FEBS Letters

Clement-Metral, Jenny D.¹  Gantt, Elisabeth¹ 

[1] Radiation Biology Laboratory, Smithsonian Institution, 12441 Parklawn Drive, Rockville, MD 20852, USA

关键词: Phycobilisome;    Photosystem II;    Oxygen evolution;    Light-harvesting antenna;    (Porphyridium cruentum);    (Red alga);    SPCM;    sucrose—phosphate—citrate—magnesium chloride medium;    chl;    chlorophyll;    PS I;    PS II;    photosystem I and II;    respectively;    LDAO;    lauryldimethylamine oxide;    DMSO;    dimethylsulfoxide;    DMBQ;    2;    6-dimethyl-p-benzoquinone;    SiMo;    12-silicomolybdate (Na4Mo12O40);    DCMU;    3-(3;    4-dichlorophenyl)-1;    1 dimethylurea;    NH2OH;    hydroxylamine;   

DOI  :  10.1016/0014-5793(83)80274-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Photosystem II phycobilisome particles were isolated from the red alga Porphyridium cruentum in a sucrose—phosphate—citrate—magnesium medium. These particles showed high rates of oxygen evolution in the water-to-silicomolybdate reaction (2300 μmol O₂.mg chl^-1.h^-1). Photosystem I, according to fluorescence emission spectra (−196°C), was greatly reduced or absent. The results demonstrate a close physical relationship of photosystem II with the phycobilisomes which are the major light harvesting antennae in this organism.

【 授权许可】

Unknown