Identification of the 3′ ‐ and 5′ ‐ends of beet necrotic yellow vein virus RNAs

FEBS Letters

Pinck, L.²  Fritsch, C.¹  Pinck, M.¹  Putz, C.² 

[1] Laboratoire de Virologie, Institut de Biologie Moléculaire et Cellulaire du CNRS, 15 rue Descartes, 67084 Strasbourg, France;Station de Pathologie Végétale, INRA, 28 rue de Herrlisheim, 68021 Colmar, France

关键词: Plant virus;    Tobamo virus;    RNA;    Cap structure;    Poly(A);   

DOI  :  10.1016/0014-5793(83)80244-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Beet necrotic yellow vein virus RNAs are characterized by a 5′ -end cap structure and polyadenylated 3′ -ends. The presence of a poly(A) sequence with major lengths ranging from 65–140 nucleotides could be demonstrated by homochromatography and sequencing methods. Beet necrotic yellow vein virus RNAs could be 5′ -end labelled with kinase only after enzymatic or chemical cap elimination followed by dephosphorylation. The 5′ -end structure is found to be of cap O type: m⁷GpppNp.

【 授权许可】

Unknown