FEBS Letters | |
Inhibition of the mitochondrial bc 1 complex by dibromothymoquinone | |
Lenaz, Giorgio1  Rugolo, Michela1  Esposti, Mauro Degli1  | |
[1] Institute of Botany, University of Bologna, Via Irnerio 42, 40126 Bologna, Italy | |
关键词: Dibromothymoquinone; Ubiquinol—cytochrome c oxidoreductase (bc1 complex); Mitochondria; Respiratory chain; Iron—sulphur protein; DBMIB; 2; 5-dibromo-3-methyl-6-isopropylbenzoquinone; DCIP; dichlorophenolindophenol; EDTA; ethylendiaminotetraacetate; EPR; electron paramagnetic resonance; Qo; 2; 3-dimethoxy-5-methylbenzoquinone; Q1; ubiquinone-1; Q1H2 Q3H2; ubiquinol-1 and ubiquinol-3; TTFA; thenoyltrifluoroacetone; UHDBT; 5-n-undecyl-4; 7-dioxobenzothiazole; | |
DOI : 10.1016/0014-5793(83)80238-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We have studied the effects of dibromothymoquinone (DBMIB) in various redox activities of the succinate—cytochrome c span of the mitochondrial respiratory chain. At concentrations higher than 50 mol/mol of cytochrome c 1 the inhibitor produces a bypass of electron transfer on the substrate side of the bc 1 complex, because of its autooxidation capability. This induces an artifactual overestimation of the real inhibition titer of the redox activity of this enzyme, which has been found to be 3–6 mol/mol of cytochrome c 1 by following the ubiquinol—cytochrome c reductase activity. This action is reversed by addition of excess of sulphydryl compounds like cysteine.
【 授权许可】
Unknown
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