Photoaffinity labeling of chloroplast cytochrome b 6‐f complex by an inhibitor azido‐derivative

FEBS Letters

Oettmeier, Walter¹  Masson, Klaus¹  Olschewski, Ellen¹ 

[1] Lehrstuhl Biochemie der Pflanzen, Ruhr-Universität, Postfach 10 21 48, 4630 Bochum 1, FRG

关键词: Photoaffinity label;    Cytochrome bin6-f complex;    Inhibitor;    Plastoquinone;    Rieske Fe-S protein;    Cytochrome b6;   

DOI  :  10.1016/0014-5793(82)80612-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The photoaffinity label 2-azido-2′,4,4′-trinitro-6-sec.butyldiphenylether inhibits photosynthetic electron transport in the thylakoid membrane as well as plastohydroquinone-plastocyanin-oxidoreductase activity in the isolated chloroplast cytochrome b ₆-f complex. In the latter, the ¹⁴C-labeled inhibitor upon UV-irradiation binds covalently only to the cytochrome b ₆ and the Rieske Fe-S peptide subunit.

【 授权许可】

Unknown