Partial purification and characterization of the glucagon receptor

FEBS Letters

Wright, David E.²  Horuk, Richard¹ 

[1] Laboratory of Cellular and Developmental Biology, Bldg. 6, Room B1-13, National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20205, USA;Chemical Research Dept., McNeil Pharmaceutical, Spring House, PA 19002, USA

关键词: Glucagon;    Hormone receptor;    N E-4-Azidophenylamidinoglucagon;    APA-glucagon;    N E-4-azidophenylamidinoglucagon;    DTT;    dithiothreitol;    CHAPS;    3-(3-cholamidopropyl)-dimethylammonio-I-propanesulfonate;    SDS—PAGE;    sodium dodecyl sulfate—polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(82)80605-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Specific labeling of liver plasma membrane glucagon receptors has been achieved by the photoincorporation of a ¹²⁵I-labeled photoderivative of glucagon, N ^E-4-azidophenylamidinoglucagon. Identification of glucagon receptors was facilitated by irradiating membranes in the presence of excess unlabeled glucagon. Isoelectric focusing of radioiodinated membrane proteins revealed one major band of glucagon displaceable material which had an isoelectric point of 5.85. When this material was isolated and run on SDS-polyacrylamide gels a major labeled band of M _r 55 000 was obtained which had properties consistent with those of the glucagon receptor. These studies indicate that a purification of the glucagon receptor of > 700-fold can be attained through the use of isoelectric focusing and SDS—polyacrylamide electrophoresis.

【 授权许可】

Unknown