Changes in nuclear content of protein conjugate histone H2A—ubiquitin during rooster spermatogenesis

FEBS Letters

Agell, Neus¹  Mezquita, Cristóbal¹  Chiva, Manel¹ 

[1] Departamento de Fisiología, Facultad de Medicina, Universidad de Barcelona, Casanova 143, Barcelona-36, Spain

关键词: Histone H2A;    Ubiquitin;    uH2A;    A24;    Chromatin;    Spermatogenesis;   

DOI  :  10.1016/0014-5793(82)80604-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Electrophoretic analysis of acid-soluble chromosomal proteins isolated from rooster testis cell nuclei at different stages of spermatogenesis, revealed that the nuclear content of a protein identified by its solubility, electrophoretic mobility and amino acid analysis as the protein conjugate histone H2A—ubiquitin (uH2A, A24) changed markedly from meiotic cells to late spermatids. The protein was not detectable in tetraploid primary spermatocytes; it was present in 1.7% of the total amount of nucleosomal core histones in early spermatids and reached its maximum level (3.5% and 11%) at the end of spermiogenesis, when histones are replaced by the protamine galline.

【 授权许可】

Unknown