A single‐step procedure for the isolation of individual mRNA species from crude lysates of Physarum polycephalum

FEBS Letters

Smulian, Nadia J.¹  Cox, Robert A.¹ 

[1] National Institute for Medical Research, Mill Hill, London NW7 1AA, England

关键词: Physarum;    Plasmoidal lysate;    Hybrid selection;    Messenger RNA;    Purification;    Poly(A)+-messenger RNA;   

DOI  :  10.1016/0014-5793(83)80212-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An individual mRNA species was isolated directly from lysates of Physarum polycephalum by hybridization to immobilised complementary DNA. The solvent for lysis was a 6 M-guanidinium salt solution, and the hybridization reaction was carried out at 42°C in 4 M guanidinium salt/33% (v/v) formamide buffer. The mRNA species isolated by this procedure was active in cell-free protein synthesis and directed the synthesis of a polypeptide of M _r 25 500 ± 750.

【 授权许可】

Unknown