Inhibition study of ADP,ATP transport in mitochondria with trinitrophenyl‐modified substrates

FEBS Letters

Boos, K.-S.²  Schlimme, E.²  Onur, G.¹  Ponse, G.¹ 

[1] Lehrstuhl Botanik II (Biochemische Pflanzenphysiologie) der Universität, Universitätsstrae 1, 4000 Düsseldorf, FRG;Laboratorium für Biologische Chemie im Fachgebiet Organische Chemie der Universität (GH), Warburger Strae 100, 4790 Paderborn, FRG

关键词: ADP;    ATP transport;    Inhibition;    2;    4;    6-Trinitrophenyl(TNP) compound;    Rat liver mitochondria;   

DOI  :  10.1016/0014-5793(83)80197-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ADP,ATP carrier of rat liver mitochondria is specifically inhibited by Meisenheimer-type trinitrophenyl (TNP) derivatives of ADP and ATP. Due to a systematic inhibition study we could show that the TNP-moiety itself, even in the 2′, 3′-O-cyclic Meisenheimer complex, revealed no inhibition of mitochondrial ADP,ATP transport. Nucleosidic TNP-compounds are weak inhibitors. Introduction of a phosphate group at the 5′-position, however, enhances the inhibitory power markedly. Our findings point to a synergistic effect of the 5′-phosphate chain and the TNP-moiety. Irreversible inhibition by TNP-nucleotides can be ruled out due to the fact that the inhibited ADP,ATP-transport system is fully reactivated by addition of albumin.

【 授权许可】

Unknown