FEBS Letters | |
Inhibition study of ADP,ATP transport in mitochondria with trinitrophenyl‐modified substrates | |
Boos, K.-S.2  Schlimme, E.2  Onur, G.1  Ponse, G.1  | |
[1] Lehrstuhl Botanik II (Biochemische Pflanzenphysiologie) der Universität, Universitätsstrae 1, 4000 Düsseldorf, FRG;Laboratorium für Biologische Chemie im Fachgebiet Organische Chemie der Universität (GH), Warburger Strae 100, 4790 Paderborn, FRG | |
关键词: ADP; ATP transport; Inhibition; 2; 4; 6-Trinitrophenyl(TNP) compound; Rat liver mitochondria; | |
DOI : 10.1016/0014-5793(83)80197-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The ADP,ATP carrier of rat liver mitochondria is specifically inhibited by Meisenheimer-type trinitrophenyl (TNP) derivatives of ADP and ATP. Due to a systematic inhibition study we could show that the TNP-moiety itself, even in the 2′, 3′-O-cyclic Meisenheimer complex, revealed no inhibition of mitochondrial ADP,ATP transport. Nucleosidic TNP-compounds are weak inhibitors. Introduction of a phosphate group at the 5′-position, however, enhances the inhibitory power markedly. Our findings point to a synergistic effect of the 5′-phosphate chain and the TNP-moiety. Irreversible inhibition by TNP-nucleotides can be ruled out due to the fact that the inhibited ADP,ATP-transport system is fully reactivated by addition of albumin.
【 授权许可】
Unknown
【 预 览 】
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