Functional groups of elongation factor 2 involved in interactions with guanosine nucleotides and ribosomes

FEBS Letters

Aktar, Neş'e Bilgin¹  Nurten, Rüstem¹  Bermek, Engin¹ 

[1] Biyofizik Bilim Dalı, Istanbul Tıp Fakültesi, Çapa, Istanbul, Turkey

关键词: Elongation factor 2;    Ribosomal interaction;    Guanosine nucleotide;    Sulfhydryl group;    N-ethylmaleimide;    Amino group specific reagent;   

DOI  :  10.1016/0014-5793(83)80189-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Treatment of rat liver EF-2 with N-ethylmaleimide (MalNEt) did not affect the direct interactions of the factor with guanine nulceotides or with ribosomes, but inhibited the binding of guanosine 5′-(β,γ-methylene)triphosphate (GuoPP(CH₂)P) to the EF-2-ribosome complex. The amino group reactive reagent 2,4,6-trinitrobenzenesulfonate (TNBS), however, inhibited specifically the direct interactions of EF-2 with guanine nucleotides, but not the binding of GuoPP(CH₂)P to the EF-2-ribosome complex. The different sensitivities of EF-2 to MalNEt and to TNBS suggested that the binding sites involved in the binary vs. ternary complex might correspond to different conformational states or might even be distinct physical entities.

【 授权许可】

Unknown