Mechanism of action of Bacillus thuringiensis var israelensis insecticidal δ‐endotoxin

FEBS Letters

Thomas, W.E.¹  Ellar, D.J.¹ 

[1] Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QW, England

关键词: Bacillus thuringiensis var israelensis;    δ-Endotoxin;    Phospholipid receptor;    Insecticide;    Mosquito;    Liposome binding;   

DOI  :  10.1016/0014-5793(83)80183-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Bacillus thuringiensis var israelensisδ-endotoxin protein active against mosquitoes was inactivated by prior incubation with lipids extracted from Aedes albopictus cells. Experiments with lipid dispersions and multilamellar liposomes showed that the toxin binds to phosphatidyl choline, sphingomyelin and phosphatidyl ethanolamine provided these lipids contain unsaturated fatty acids. Phosphatidyl serine binds toxin less efficiently and phosphatidyl inositol, cardiolipin, cerebroside and cholesterol show no affinity for the toxin. The results suggest an insecticidal mechanism in which interaction of toxin with specific plasma membrane lipids causes a detergent-like rearrangement of the lipids, leading to disruption of membrane integrity and eventual cytolysis.

【 授权许可】

Unknown