Structure of DNA in metaphase chromosomes of mouse fibroblasts

FEBS Letters

Belyaev, N.D.¹  Budker, V.G.¹  Dubrovskaya, V.A.¹  Matveeva, N.M.¹ 

[1] Novosibirsk Institute of Organic Chemistry, Siberian Branch of USSR Academy of Sciences, Novosibirsk 630090, USSR

关键词: Chromosomes;    Chromatin;    Methylation;    DNA structure;    DMS;    dimethylsulphate;    Tris;    tris-hydroxymethylaminomethane;    EDTA;    ethylenediaminotetraacetic acid;   

DOI  :  10.1016/0014-5793(83)80167-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We show tht N-1 in adenine of chromosomal DNA is methylated by treatment of metaphase chromosomes with dimethylsulphate while this is not the case in chromatin. The data on methylation are consistent with those obtained from the experiments with S₁-nuclease treatment of chromatin and chromosomes. This suggests a disarrangement of DNA secondary structure in the metaphase chromosomes.

【 授权许可】

Unknown