Evidence that amiloride antagonises insulin‐stimulated protein phosphorylation by inhibiting protein kinase activity

FEBS Letters

Hardie, D.Grahame¹  Holland, Ross¹  Woodgett, James R.¹ 

[1] Biochemistry Department, Dundee University, Medical Sciences Institute, Dundee DD1 4HN, Scotland

关键词: Insulin;    Protein phosphorylation;    Amiloride;    Protein kinase;   

DOI  :  10.1016/0014-5793(83)80163-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The diuretic drug amiloride antagonises the insulin-dependent increase in phosphorylation of ATP-citrate lyase in hepatocytes isolated from rats that had been starved and refed a fat-free diet. Studies with a range of protein kinases and protein phosphatases that have been shown to phosphorylate or dephosphorylate purified ATP-citrate lyase in vitro revealed that amiloride was a non-specific inhibitor of all protein kinases tested, but did not significantly affect any of the protein phosphatases. These results cast doubt on previous claims that growth factors stimulate phosphorylation of ribosomal protein S6 by activating an amiloride-sensitive Na⁺/H⁺ exchange system, and that insulin inhibits a protein phosphatase that is activated by amiloride.

【 授权许可】

Unknown