Platelet activating factor (PAF‐acether) promotes an early degradation of phosphatidylinositol‐4,5‐biphosphate in rabbit platelets

FEBS Letters

Mauco, Gérard¹  Louis, Douste-Blazy¹  Hugues, Chap¹ 

[1] INSERM Unité 101, Biochimie des Lipides, Hôpital Purpan, 31059 Toulouse, France

关键词: Platelets;    Platelet activating factor;    (poly)Phosphoinositides;    phosphatidic acid;    Phosphomonoesterase;   

DOI  :  10.1016/0014-5793(83)80643-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

³²P-Labelled washed rabbit platelets were incubated with 0.6 nM platelet activating factor (PAF-acether), giving a full aggregation and release response within 30–60 s. The major phospholipid changes observed under these conditions were: (1) An increased labelling of phosphatidic acid (PA) within 10 s and of phosphatidylinositol (MPI) at 30 s, reflecting the activation of the MPI cycle via the cytosolic phospholipase C; (2) an enhancement of phosphatidylinositol-4-phosphate (DPI) and phosphatidylinositol-4,5-bisphosphate (TPI) labelling at later incubation times; (3) an early degradation of TPI with a counterbalancing formation of DPI. The latter changes suggest a receptor-mediated stimulation of TPI-phosphomonoesterase, the role of which in the mechanism of platelet activation is discussed.

【 授权许可】

Unknown