Encapsulation of hemoglobin in phospholipid vesicles

FEBS Letters

Yager, Paul¹  Gaber, Bruce P.¹  Chang, Eddie L.¹  Sheridan, James P.¹ 

[1] Code 6512, Bio/molecular Optics Section, Optical Probes Branch, Naval Research Laboratory, Washington, DC 20375, USA

关键词: Hemoglobin;    Phospholipid;    Vesicles;    Liposomes;    Encapsulation;    Blood;   

DOI  :  10.1016/0014-5793(83)80625-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hemoglobin has been encapsulated in phospholipid vesicles by extrusion of hemoglobin/lipid mixtures through polycarbonate membranes. This technique avoids the use of organic solvents, sonication, and detergents which have proven deleterious to hemoglobin. The vesicles are homogeneous, with a mean size of 2400 Å as determined by photon correlation spectroscopy. The encapsulated hemoglobin binds oxygen reversibly and the vesicles are impermeable to ionic compounds. Hemoglobin encapsulated in egg phosphatidylcholine vesicles converts to methemoglobin within 2 days at 4°C. By contrast, when a mixture of dimyristoyl phosphatidylcholine, cholesterol and dicetyl phosphate is used there is no acceleration in methemoglobin formation, and the preparation is stable for at least 14 days at 4°C.

【 授权许可】

Unknown