Purification and partial characterization of α‐pheromone‐binding protein from a mating‐type cells in the yeast Saccharomyces cerevisiae

FEBS Letters

Yoshida, Kazuo¹  Shimizu, Tatsuro¹  Fujimura, Hiroaki¹  Yanagishima, Naohiko¹ 

[1] Biological Institute, Faculty of Science, Nagoya University, Chikusa-ku, Nagoya 464, Japan

关键词: Binding protein;    α-Pheromone;    Sexual agglutinability;    Receptor;    Saccharomyces cerevisiae;    Yeast;   

DOI  :  10.1016/0014-5793(83)80110-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two kinds of proteinacious substances which bind to α pheromone were isolated from heat-shock extract of a mating type cells of Saccharomyces cerevisiae. One has M _r ∼300 000 and the other M _r 58 000. We purified the smaller binding protein by successive application of DEAE and CM-cellulose chromatography, gel filtration, and isoelectrofocusing. The purified binding protein showed a single band by polyacrylamide gel electrophoresis. This binding protein was detected in a cells but not in α cells.

【 授权许可】

Unknown