Rapid conversion of newly‐synthesized orotate to uridine‐5‐monophosphate by rat liver cytosolic enzymes

FEBS Letters

Dileepan, Kottarappat N.¹  Kennedy, James¹ 

[1]Enzymology Research Laboratory, Veterans Administration Medical Center, 4801 Linwood Boulevard, Kansas City, MI 64128, USA

关键词: Dihydroorotate dehydrogenase;    Effect of cytosol;    Apparent inhibition;    Orotate;    Pyrimidine;    Mitochondria;    DHO-DHase;    dihydroorotate dehydrogenase (EC 1.3.3.1);    ODCase;    orotidylate decraboxylase (EC 4.1.1.23);    OPRTase;    orotate phosphoribosyltransferase (EC 2.4.2.10);    PRPP;    5-phosphorylribose-1-pyrophosphate;    UMP;    uridine-5-monophosphate;   

DOI  :  10.1016/0014-5793(83)80107-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It had been noted previously that the activity of mitochondrial dihydroorotate dehydrogenase was lower in crude tissue preparations containing cytosol than in isolated mitochondria. Closer examination reveals that the apparent lower enzyme activity is due to rapid conversion of newly-synthesized orotate to uridine-5-monophosphate by the cytosolic enzymes, orotate phosphoribosyltransferase and orotidylate decarboxylase.

【 授权许可】

Unknown