Effect of trifluoperazine on 3H‐labeled protein secretion induced by pentoxifylline, cholinergic or adrenergic agonists in rat lacrimal gland. A possible role of calmodulin?

FEBS Letters

Mauduit, Philippe¹  Herman, Geneviève¹  Rossignol, Bernard¹ 

[1] Laboratoire de Biochimie des Transports Cellulaires, Institut de Biochimie, Bât 432, Université de Paris-Sud, 91405 Orsay Cedex, France

关键词: Calmodulin;    Calcium;    Lacrimal gland;    Protein secretion;    Trifluoperazine;   

DOI  :  10.1016/0014-5793(83)80381-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In rat lacrimal gland, cholinergic, α- or β-adrenergic or methylxanthine stimulations of protein secretion are extracellular calcium dependent. 10 μM trifluoperazine (TFP) inhibited only cholinergic and α-adrenergic stimulations. Half maximal effect was observed at 30 μM, with all inducers except norepinephrine (3 μM). 10 or 30 μM TFP also suppressed the decrease of L-[³H]leucine incorporation into protein due to carbamylcholine. 100 μM TFP inhibited protein secretion and L-[³H]leucine incorporation. 500 μM TFP promoted cell lysis. It is suggested that: (a) at 100 μM TFP, inhibition is not specific for protein secretion; (b) at 30 μM TFP, inhibition could be related to a role of calmodulin in the secretory regulation process.

【 授权许可】

Unknown