Purification of a troponin I‐like factor from pig platelet

FEBS Letters

d'Albis, A.¹  Pruliere, G.¹  Terrossian, E.der¹ 

[1] Laboratoire de Biologie Physicochimique, Bât. 433, Université Paris-Sud, 91405 Orsay, France

关键词: Pig platelet;    Troponin I-like factor;    Actin-linked regulation;    Calmodulin;    Tropomyosin;    Calcium sensitivity;    PAGE;    polyacrylamide gel electrophoresis;    TLCK;    tosyl lysyl chloromethyl ketone;    EGTA;    ethylene glycol-bis (β-aminoethylether)-N;    N′)-tetraacetic acid;    EDTA;    ethylenediamine tetraacetic acid;   

DOI  :  10.1016/0014-5793(83)80380-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A troponin I-like factor has been purified from pig platelet by G150 Sephadex filtration of a low ionic strength extract, acidification at pH 4.2, ion exchange on DE-52 cellulose, and affinity chromatography on calmodulin-Sepharose. This protein (M _r 17000), together with pig brain calmodulin and platelet tropomyosin, is able to participate to the reconstitution in vitro of a thin filament-like complex which modulates with 55% calcium sensitivity ¹ the platelet actin-activated Mg²⁺-dependent ATPase activity of rabbit skeletal muscle myosin.

【 授权许可】

Unknown