Dihydropyridines as potent calcium channel blockers in neuronal cells

FEBS Letters

Takahashi, Masami¹  Ogura, Akihiko¹ 

[1] Laboratory of Neurochemistry, Mitsubishi-Kasei Institute of Life Sciences, Machida-shi Tokyo 194, Japan

关键词: Neuronal Ca2+ channel;    Dihydropyridines;    Transmitter release;    Ca2+ uptake;    Radioligand binding;    Molecular probe;    BSS;    balanced salt solution;    DMEM;    Dulbecco's modified Eagle's medium;    GABA;    γ-aminobutyric acid;    Hepes;    N-2-hydropxyethylpiperazine N′-ethanesulfonic acid;    NE;    norepinephrine;    Tris;    tris (hydroxymethyl) aminomethane;   

DOI  :  10.1016/0014-5793(83)80377-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nicardipine, one of the dihydropyridine derivatives, in a nanomolar concentration range suppressed the high K⁺-induced neurotransmitter from cultured neuronal cells (chick embryonic neural retina cells and clonal rat pheochromocytoma cells). The high K⁺-induced Ca²⁺ uptake into pheochromocytoma cell was also blocked by nicardipine in the same concentration range. [³H]Nitrendipine, another dihydropyridine derivative, bound specifically to pheochromocytoma cell homogenate in a saturable manner. We concluded that dihydropyridines block and bind to the high K⁺-sensitive Ca²⁺ channels in neuronal cells.

【 授权许可】

Unknown