The effect of a ribonuclease inhibitor from human placenta on the in vitro synthesis of human placental proteins

FEBS Letters

Kusamran, T.¹  Hiranyavasit, W.¹ 

[1]Department of Biochemistry, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand

关键词: Human placental RNA;    Placental ribonuclease inhibitor;    hPL;    hCG;    In vitro translation;    Wheat germ;   

DOI  :  10.1016/0014-5793(83)80476-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Addition of a ribonuclease inhibitor (10 μg/ml) from human placenta caused 2–3 fold increase of [³H]leucine incorporation in the wheat germ extract as directed by human placental poly (A)-mRNA. Analysis of the translated products by sodiumdodecylsulfate/polyacrylamide gel electrophoresis/fluorography revealed that the inhibitor preferentially increased the yields of the larger proteins, particularly those of larger than M _r 40 000. In the presence of the inhibitor, yields of two placental proteins (human placental lactogen and human chorionic gonadotropin) were increased about 70–80% as detected by immunoprecipitation with specific homologous antisera. The method provided an improvement of translation system for studying biosynthesis of other human placental proteins.

【 授权许可】

Unknown