Proteolysis of Acinetobacter citrate synthase by subtilisin

FEBS Letters

Mitchell, Colin G.¹  Weitzman, P.D.J.¹ 

[1] Department of Biochemistry, University of Bath, Bath BA2 7AY, England

关键词: Citrate synthase;    Acinetobacter calcoaceticus Proteolysis;    Subtilisin;    Cooperativity;    Regulation;   

DOI  :  10.1016/0014-5793(83)80083-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Citrate synthase from Acinetobacter calcoaceticus was subjected to proteolysis with subtilisin. Although the enzyme proved relatively resistant to inactivation by this treatment, SDS-polyacrylamide gel electrophoresis clearly revealed breakdown of the citrate synthase to smaller fragments. The regulatory responses of the native enzyme to inhibition by NADH and re-activation by AMP were retained on proteolysis, indicating that the fragments bind tightly to each other and preserve the overall cooperative molecular interactions.

【 授权许可】

Unknown