Alternative hypotheses of proton ejection in cytochrome oxidase vesicles

FEBS Letters

Moyle, Jennifer¹  Mitchell, Peter¹ 

[1] Glynn Research Institute, Bodmin, Cornwall, PL30 4AU, England

关键词: Cytochrome oxidase vesicles;    Redox-linked proton pump;    Redox-linked deprotonation;    DCCD-sensitive phosphatidyl serine-cytochrome c complex;    Redox pulse;    Proton ejection;    Δe−;    quantity of electrons transferred in redox pulse;    Δϕ;    electric membrane potential;    Bo;    pH buffering powers of outer;    B1;    inner aqueous media;    obs;    observed;    tot;    total;    pmp;    pump;    dep;    deprotonation;    err;    error;    val;    valinomycin;    CCCP;    carbonylcyanide m-chlorophenyl-hydrazone;    FCCP;    carbonylcyanide p-trifluoromethoxy-phenylhydrazone;    DCCD;    N;    N'-dicyclohexyl-carbodimide;    TMPD;    N;    N;    N';    N'-tetramethyl-p-phenylenediamine;   

DOI  :  10.1016/0014-5793(83)80063-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A review of published experimental and interpretative knowledge concerning proton ejection associated with cytochrome c oxidation by artificial phospholipid vesicles inlaid with cytochrome c oxidase indicates that the detailed characteristics of the redox-linked proton ejection cannot be simply explained by proton pumping. We propose an alternative hypothesis according to which proton ejection is due to the redoxlinked deprotonation of a complex involving phospholipid and cytochrome c at the surface of the vesicles. The postulates upon which this hypothesis depends are explicitly outlined, and some methods of testing the hypothesis are suggested.

【 授权许可】

Unknown