The substructure of phosphodiesterase as established by radiation inactivation

FEBS Letters

Ellory, J.C.²  Klein, R.A.³  Ottolenghi, P.¹ 

[1] Institute of Physiology, University of Aarhus, 8000 Aarhus, Denmark;Physiological Laboratory, University of Cambridge England;Medical Research Council, Molteno Institute, University of Cambridge, Downing Street, Cambridge CB2 2EE, England

关键词: Phosphodiesterase;    Calmodulin;    Radiation inactivation;    Dimerisation;   

DOI  :  10.1016/0014-5793(82)80758-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A model for the activation of phosphodiesterase by calmoduling based on a conversion of inactive dimers to active monomers, derived from radiation inactivation studies J. Biol. Chem. (1981) 256, 11351–11355 has been re-examined using a simple probability argument. We conclude that the original model is not supported by the radiation inactivation studies, since our analysis of this model would predict that the rate of radiation inactivation of calmodulin-dependent phosphodiesterase activity be exactly twice that for the decay in total activity in marked contrast with the results obtained.

【 授权许可】

Unknown