Inhibition of the T7 DNA polymerase by insulin

FEBS Letters

Randahl, Håkan¹ 

[1] Department of Chemistry, Karolinska Institutet, S-104, Stockholm, Sweden

关键词: T7 DNA polymerase;    Thioredoxin;    Thioredoxin reductase;    Insulin;   

DOI  :  10.1016/0014-5793(82)81314-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

T7 DNA polymerase reduced insulin at the same K _m as thioredoxin, while the turnover number decreased. Recycling of the disulfide of thioredoxin subunit to its dithiol form was made by thioredoxin reductase. Incubation of T7 DNA polymerase with insulin decreases its ability to bind DNA and therefore inhibited polymerase and exonuclease activities. Thioredoxin reductase fully reversed this inhibition. Insulin did not induce dissociation of the T7 DNA polymerase subunits, which was tested by immunoadsorbent chromatography. No significant difference in single-stranded exonuclease compared to polymerase activity was seen in the flow through or the eluate, which had been expected if a dissociation of the subunits had occurred.

【 授权许可】

Unknown