Hydrophobic chromatography of fibronectin

FEBS Letters

Morgenthaler, J.-J.¹ 

[1] Central Laboratory, Swiss Red Cross Blood Transfusion Service, Wankdorfstrasse 10, CH-3000 Bern 22, Switzerland

关键词: Fibronectin;    Hydrophobic interaction;    Chromatography;    Alkane—Sepharose;    Proteolysis;    SS-bridges;    PBS;    phosphate-buffered saline;    DTT;    dithiothreitol;    PMSF;    benzyl sulfonyl fluoride;    SDS;    sodium dodecyl sulfate;    PAGE;    polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(82)81308-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fibronectin was chromatographed on immobilized alkanes of various chain lengths. No binding of the protein to the hydrophobic matrix was observed with alkanes containing from 3–7 methylene groups; the protein bound, however, to immobilized alkanes with 8 or 10 methylene groups. Fibronectin could be quantitatively eluted from commercial Octylsepharose with a non-ionic detergent. A gelatin-based plasma expander did not interfere with the binding. Many proteolytic fibronectin fragments also bound to a hydrophobic matix. The results show that fibronectin posesses at least one hydrophobic binding site.

【 授权许可】

Unknown