Effects of eicosatetraynoic acid (ETYA) on cultured pig thyroid cells

FEBS Letters

Jacquemin, Claude¹  Gerard, Corinne¹  Haye, Bernard¹ 

[1] Laboratoire de Biochimie, UER Sciences, BP 347, 51062 Reims-Cédex, France

关键词: Eicosatetraynoic acid;    Arachidonic acid;    Porcine thyroid cell;    Thyrotropin;    Cyclic AMP;    Phospholipid;   

DOI  :  10.1016/0014-5793(82)80825-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The arachidonate inhibition of the adenylate-cyclase system of cultured pig thyroid cells was not mediated by cyclooxygenase, lipoxygenase or peroxidase metabolites. Indeed ETYA, an inhibitor of cyclooxygenase and lipoxygenase, and methimazole, an inhibitor of peroxidase and iodination were without effect on the arachidonate inhibition. Moreover the effect of arachidonate was amplified by a combination with ETYA. In ³²P incorporation experiments we observed a modification of the labelling of individual phospholipids of cultured pig thyroid cells resulting in a decrease into phosphatidylinositol (PI) and an increase into phosphatidate (PA) of arachidonate and ETYA-treated cells. These results may be explained by an inhibition of CDP-diacylglycerol: inositol transferase and conversely a stimulation of PI specific phospholipase C yielding a decrease in PI and an increase in PA, which inhibits in turn adenylate cyclase activity possibly by Ca²⁺ translocation.

【 授权许可】

Unknown