Separation and chemical differentiation of α and β subunits in yeast phosphofructokinase

FEBS Letters

Yon, Jeannine M.¹  Chaffotte, Alain F.¹  Laurent, Michel¹  Tijane, M'hammed N.¹ 

[1] Laboratoire d'Enzymologie Physico-chimique et Moléculaire, Groupe de Recherche no. 13 du Centre National de la Recherche Scientifique, associé à l'Université de Paris-Sud, Centre d'Orsay, 91405 Orsay, France

关键词: Phosphofructokinase;    Glycolysis;    Allostery;    Catalytic subunit;    Regulatory subunit;    Protein chemistry;   

DOI  :  10.1016/0014-5793(82)80822-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The two types of subunits α and β constitutive of yeast phosphofructokinase have been separated by ion-exchange chromatography under denaturating conditions. Amino acid analysis and peptide mapping were performed on the isolated subunits. The frequence of most of the amino acids significantly differs between the two types of polypeptide chains. Moreover, tryptic peptide maps of α and β subunits are clearly not superimposable. These chemical differences seem sufficient to account for the distinct catalytic and regulatory functions of β and α subunits in the yeast phosphofructokinase reaction.

【 授权许可】

Unknown