Carbodiimide‐induced protein—RNA crosslinking in mammalian ribosomal subunits

FEBS Letters

Buisson, M.¹  Reboud, A.M.¹ 

[1] Laboratoire de Biochimie Médicale, UER Lyon Nord, Université Lyon I, 43 Bd du 11 novembre, 69622 Villeurbanne Cedex, France

关键词: Mammal;    Ribosome;    Crosslinking;    Carbodiimide;    Protein;    RNA;   

DOI  :  10.1016/0014-5793(82)80817-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

RNA—protein interactions in the 60 S subunits of rat liver ribosomes were studied using 1-ethyl-3-dimethylaminopropyl carbodiimide (EDC) under conditions which neither changed the sedimentation coefficient of subunits nor the intactness of their rRNA. EDC induced RNA—protein and protein—protein crosslinkings. Proteins crosslinked to 28 S RNA were identified by two-dimensional gel electrophoreses as L17, L19, L23a and L37a, shown to react with 28 S RNA when using a low dose of UV radiation. Attempts have also been made to use EDC for the studies of RNA—protein interactions in 40 S ribosomal subunits.

【 授权许可】

Unknown