期刊论文详细信息
FEBS Letters | |
A biotin‐dependent sodium pump: glutaconyl‐CoA decarboxylase from Acidaminococcus fermentans | |
Buckel, Wolfgang1  Semmler, Roswitha1  | |
[1] Biochemie I, Universität Regensburg, D-8400 Regensburg, FRG | |
关键词: Glutaconyl-CoA decarboxylase; Biotin; Sodium ion transport; Avidin; Triton X-100; Monensin; | |
DOI : 10.1016/0014-5793(82)81237-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The decarboxylation of glutaconyl-CoA to crotonyl-CoA in the anaerobic bacterium Acidaminococcus fermentans is catalysed by a membrane-bound, biotin-dependent enzyme which requires Na+ for activity. Inverted vesicles from A. fermentans accumulated Na+ only if glutaconyl-CoA was decarboxylated. The Na+ uptake was inhibited by avidin but not by the avidin biotin complex. Detergents and ionophores such as monensin also prevented the Na+ transport. The results indicate that the enzyme is able to convert the free energy of decarboxylation (ΔG o′ ≈−30 kJ/mol) into a Na+ gradient.
【 授权许可】
Unknown
【 预 览 】
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