A biotin‐dependent sodium pump: glutaconyl‐CoA decarboxylase from Acidaminococcus fermentans

FEBS Letters

Buckel, Wolfgang¹  Semmler, Roswitha¹ 

[1] Biochemie I, Universität Regensburg, D-8400 Regensburg, FRG

关键词: Glutaconyl-CoA decarboxylase;    Biotin;    Sodium ion transport;    Avidin;    Triton X-100;    Monensin;   

DOI  :  10.1016/0014-5793(82)81237-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The decarboxylation of glutaconyl-CoA to crotonyl-CoA in the anaerobic bacterium Acidaminococcus fermentans is catalysed by a membrane-bound, biotin-dependent enzyme which requires Na⁺ for activity. Inverted vesicles from A. fermentans accumulated Na⁺ only if glutaconyl-CoA was decarboxylated. The Na⁺ uptake was inhibited by avidin but not by the avidin biotin complex. Detergents and ionophores such as monensin also prevented the Na⁺ transport. The results indicate that the enzyme is able to convert the free energy of decarboxylation (ΔG ^o′ ≈−30 kJ/mol) into a Na⁺ gradient.

【 授权许可】

Unknown